Great Preparation
EASYECL CHEMILUMINESCENT SUBSTRATES
One Component Kits for error free results
GreatPrep Easy ECL Kit ranges
Good Measurements needs Great preparation. Introducing” GreatPrep” a range of premium, yet affordable products for Western blot workflow with futuristic technologies. Great Prep provides easy to use and error free sample preparation, thus offering convenient solution for your western blot applications.
GreatPrep EasyECL (Enhanced Chemiluminescent) reagents are non-isotopic, chemiluminescence substrates, designed for the chemiluminescent detection of immobilized proteins and immobilized nucleic acids conjugated with horseradish peroxidase (HRP).
EasyECL products are available for different levels of sensitivity to cover all detection needs from picograms to low femtograms.
Our premixed solution based on a patent-pending technology enables an increased experimental consistency avoiding pipetting errors and possible contaminations.
- Western blotting detection of HDAC-1 on Hela cell lysate with EasyECL Basic.
- Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5 µg to 0.078 µg of total protein.
- Membrane: Trans-Blot Turbo™ Mini Nitrocellulose Transfer Packs (BIO-RAD).
- Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T.
- Primary antibody: Rabbit-anti Human HDAC-1 (abcam) 1:2000.
- Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam) 1:20000.
- Detection level: Mid-picograms
- Western blotting detection of HDAC-1 on Hela cell lysate with EasyECL Ultra.
- Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 5 µg to 0.078 µg of total protein.
- Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (BIO-RAD)
- Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T.
- Primary antibody: Rabbit-anti Human HDAC-1 (abcam) 1:5000.
- Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam) 1:75000.
- Detection level: Mid-femtograms
- Western blotting detection of HDAC-1 on Hela cell lysate with Easy ECL Premium.
- Sample: Two-fold dilution series of Hela whole cell
- lysate (abcam®) from 5 µg to 0.078 µg of total
- protein. Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose
- Transfer Packs (BIO-RAD).
- Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T.
- Primary antibody: Rabbit-anti Human HDAC-1 (abcam) 1:7500.
- Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:100000.
- Detection level: Mid to Low femtograms
- Western blotting detection of HDAC-1 on Hela cell lysate with EasyECL Supreme.
- Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 2,5 µg to 0.039 µg of total protein.
- Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (BIO-RAD).
- Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T.
- Primary antibody: Rabbit-anti Human HDAC-1 (abcam) 1:10000.
- Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:200000.
- Detection level: Low femtograms
- One Component – premixed solution increases the uniformity of the experiment by preventing pipetting mistakes and potential contaminations.
- One Component- Premixed solution helps to get error free results.
- Storage: Allreagents can be stored at room temperature (18-25°C).
- Shelf life: 1 year
UVITEC-Authentic Imaging specalist
Introducing IRIS – Revolutionary western blot and gel documentation imaging system.
The new Alliance IRIS is the latest generation top end imaging system on the market for chemiluminescence and fluorescence Western blots.
The standalone design of IRIS with an impressive touch screen size of over 15.6 inches is a testament to its practicality and user friendliness.
In the spirit of a sustainable environment, UVITEC Cambridge is the first to introduce UV-LED Pad technology to ensure a smooth transition from traditional UV Transilluminators.
Enjoy our 1-click acquisition process with fully automatic exposure, lighting and focus mode allows you to get outstanding quantifiable images.
IRIS integrates an unprecedented technology in the world of molecular imaging and sets up your data as never seen before.